sigma D2650二甲基亞砜使用說明

應(yīng)用DMSO 是一種用于化學(xué)反應(yīng)（如聚合酶鏈?zhǔn)椒磻?yīng) (PCR)）的極性非質(zhì)子溶劑，也可作為冷凍保護(hù)玻璃化劑用于保存細(xì)胞、組織和器官。DMSO 可在細(xì)胞冷凍培養(yǎng)基中用于保護(hù)細(xì)胞免受冰晶導(dǎo)致的機(jī)械損傷。它可用于原代、繼代培養(yǎng)和重組的異倍體和雜交瘤細(xì)胞系、胚胎干細(xì)胞 (ESC) 以及造血干細(xì)胞的凍存。DMSO 常與 BSA 或胎牛血清 (FBS) 一同使用。包裝5mL 和 10mL 火焰封口安瓿瓶包裝，100mL 棕色瓶包裝其他說明該產(chǎn)品是一種Hybri-Max產(chǎn)品。它經(jīng)過了雜交瘤測試并對其在細(xì)胞冷凍中的適用性進(jìn)行了評估。該產(chǎn)品經(jīng)無菌過濾（0.2微米）及內(nèi)毒素水平檢測。注意容易過度冷卻，并在室溫下緩慢融化固化的產(chǎn)品放在室溫下可以再次液化，對其本身無影響。常見問題 What is the molarity of DMSO? DMSO will have a molarity of 14.1M, based on a density of 1.1 g/mL and a molecular weight of 78.13 g/mol.  What is the DMSO dissolved in? DMSO is not dissolved in any solvent. It is a pure chemical compound that is a liquid at room temperature.  Where does DMSO come from? DMSO is known to have been isolated from tree bark in the past. It is now a commericially synthesized solvent.  What concentration of DMSO do I use in a cell freezing media? DMSO is usually used at 1-10%, depending on the cell line.  For the cell freezing media that we offer, it is used at 8.7% concentration.  Does DMSO have any synthetic utility? DMSO has been used to oxidize thiols and disulfides to sulfonic acids, hydrolysis of epoxides, thioalkylation of phenols, and oxidation of primary alcohols and halides, and esters of primary alcohols to aldehydes.  Which document(s) contains shelf-life or expiration date information for a given product? If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.  What is the specification for 'pass' for cell freezing on the Certificate of Analysis for Dimethyl sulfoxide (DMSO)? Product D2438 is assessed for cell freezing.  Cells are frozen and stored at -70°C.  The cells are thawed, cultured and viability is assessed at 24 hours.  There is no evidence of cytotoxicity or abnormal cell growth.  Dimethyl sulfoxide (DMSO) should be a liquid.  Why did it arrive as a solid? DMSO supercools easily, and re-melts slowly at room temperature. The melting point is 16-19 °C. The solidified product can be re-liquefied by warming to room temperature without detriment to the product.  What is the basis for the endotoxin specification for Dimethyl sulfoxide (DMSO)? The industry standard for 'endotoxin free' is less than 1 EU/mL.  What type of filter is used to sterile-filter Dimethyl sulfoxide (DMSO)? DMSO can be sterile-filtered through a 0.2 micron PTFE filter. The sterility testing is per the current US Pharmacopoeia method.  How do I get lot-specific information or a Certificate of Analysis? The lot specific COA document can be found by entering the lot number above under the "Documents" section.  How do I find price and availability? There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.  What is the Department of Transportation shipping information for this product? Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. My question is not addressed here, how can I contact Technical Service for assistance? Ask a Scientist here.